Part:BBa_K1364011:Design

Pveg-spoVG + EcAMP-1 + Double terminator

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

This part is composed of the constitutive promoter P_veg and strong RBS for B. subtilis(https://parts.igem.org/Part:BBa_K733013), the open reading frame of EcAMP-1 and a double terminator https://parts.igem.org/Part:BBa_B0015).

The EcAMP-1 part was codon optimized for E. coli by the iGEM Utah State team and thanks to the Life Technologies GeneArt software program.

Source

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